Paramagnetic Relaxation Enhancement NMR: Troponin Dynamics & Disease
We use paramagnetic relaxation enhancement (PRE)-NMR spectroscopy to describe not only structure, but also dynamics of ensemble states of the cardiac Troponin complex. PRE-NMR requires the placement of spin labels in the protein backbone which then allows us to measure long range distances between the spin label and amide groups up to ~25 Å.
Nanodiamonds: New Biolabels for Tracking & Imaging in vivo
Colour centres in nanodiamonds have many properties such as chemical and physical stability, biocompatibility, straightforward surface functionalisation as well as bright and stable photoluminescence, which make them attractive for biological applications. We are developing methods to process detonation nanodiamonds to use for the imaging and tracking of targeted biomolecules in complex biological systems.
The CLIC Ion channel family : Structural gymnastics
Chloride intracellular channel proteins (CLICs) differ from most ion channels as they can exist in both soluble and integral membrane forms. The CLICs are expressed as soluble proteins but can reversibly autoinsert into the membrane to form active ion channels.